Rare earth cryptates and homogeneous fluoroimmunoassays with human sera
نویسندگان
چکیده
منابع مشابه
Rare earth cryptates and homogeneous fluoroimmunoassays with human sera.
The rare earth cryptates are used as long-lived fluorescent labels. They are formed by inclusion of a europium ion in the intramolecular cavity of a macropolycyclic ligand containing bipyridine groups as light absorbers. The use of fluoride ions in the measuring medium allows a total shielding of the label fluorescence. Here, I have established the conditions under which the signal of these cry...
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A homogeneous assay method based on the long-lived fluorescence of rare earth cryptates and amplification by nonradiative energy transfer has been developed for immunoassays. The principles of the assay allow a double discrimination of the emitted signal through spectral and temporal selectivity. The cage structure of the complex, ion pairing around europium, as well as double-wavelength detect...
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Objective(s): Streptokinase (SKa) is an antigenic protein which is secreted by Streptococcus pyogenes. Streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. In the present study, recombinant streptokinase from S. pyogenes was produced and showed that recombinant SKa protein was recognized by infected human sera using Western blot analy...
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Five rare earth complexeslanthanum(III),praseodymium(III),neodymium(III), samarium(III) and europium(III) have been synthesized from Schiff base ligand (N,N-bis (2-hydroxy-1-naphthylidene) acetylhydrazone). The complexes were characterized based on elemental analysis, molar conductance, ultraviolet, infrared, mass, thermogravimetric and powder X-ray diffraction studies. Infrared spectra sug...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1993
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/39.9.1953